News Item
NIH Grant for DNA Sequencing Technology Development Awarded to Amit Meller's Group, Rowland Institute at Harvard
Description of Research
Ultra Fast Nanopore Readout Platform for Designed DNAs
 Amit Meller
Rowland Institute at Harvard, Harvard University, Cambridge, Mass.
We describe a novel methodology for rapid and massively parallel DNA sequencing that promises to considerably reduce the time
and cost of genome sequencing. The method includes two main steps: 1. Conversion of the target DNA molecules into easily
readable code units (Designed DNAs, invented by LingVitae AS); and 2. rapid readout of the designed DNAs using our nanopore
based approach. The first step has been recently demonstrated by LingVitae AS. Here we present a novel readout platform based
on the simultaneous optical probing of multiple nanopores.
The unique combination of designed DNAs with the nanopore optical readout, eliminates the uncertainties associated with the
development of new chemical compounds, required in other approaches. In addition, since the nanopore readout does not rely on
the relatively slow enzymatic incorporation of nucleotides, and because it can be applied to read the sequence of single molecules,
an extremely high throughput is expected, resulting in a cheaper and faster approach. In this proposal, we lay down a
straightforward experimental strategy for testing our approach, based on our expertise in nanopores and in the optical
probing of single-biomolecules.
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